Enzyme concentration
Theoretically the relationship between enzyme concentration and it's activity (usually measured as a rate by looking at how quickly a product is being made) is simple. It is directly proportional. This stands to reason. If you have twice as many enzymes you are twice as likely to get a successful collision and therefore to form an enzyme substrate complex and ultimately product. This is shown on the diagram above as a dashed line.
I reality this only holds true to a certain extent. As the concentration increases the benefit of adding more enzyme becomes less. This is because although you may add more enzymes they cannot always bind to the substrate because there may already be an enzyme bound to it. Eventually we will reach a point were all the actives sites are full and there are still 'spare' enzymes. we say the solution has reached 'saturation point' and no matter how much enzyme we add the rate won't get any quicker. This situation is represented by the solid line on the diagram above.
I reality this only holds true to a certain extent. As the concentration increases the benefit of adding more enzyme becomes less. This is because although you may add more enzymes they cannot always bind to the substrate because there may already be an enzyme bound to it. Eventually we will reach a point were all the actives sites are full and there are still 'spare' enzymes. we say the solution has reached 'saturation point' and no matter how much enzyme we add the rate won't get any quicker. This situation is represented by the solid line on the diagram above.